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Louis Pasteur is a known microbiologist with his contribution to vaccination, pasteurization as well as microbial fermentation. He has shown remarkable breakthroughs in the prevention of diseases as well as its cause. Louis Pasteur worked for puerperal fever as well as he created vaccines for anthrax as well as rabies. He repeated the experiment of spontaneous generation with a modified glass flask with a curved neck. Microorganisms were prevented from entering inside the flask due to its curved-neck and the boiled broth remained clear. Pasteur experiments on spontaneous generation were contributed to the methodology of microbiology, understanding of the origin of life and techniques for the preservation of food. He had a great contribution in the methodology of microbiology by studying the mechanism of alcohol fermentation.
He introduced other optional methods for fermentation of beer and other juice. He also worked on tartrate metabolism. He also found that alcoholic fermentation was catalyzed by the living organisms which are yeast cells. After his discoveries about fermentation Pasteur was not in favour of spontaneous generation. He also gave concepts that putrefying materials are already present in the air and this process occurs due to the microorganisms present in the atmosphere.. He supported his objection of spontaneous generation by his experiment conducted in the swan necked flask, Pasteur flask.
Pasteur also gave the concept of sterilization which is the elimination and killing of contaminants and other microorganisms from an object. Pasteur also worked on several sterilization procedures used in future basic and applied science. Pasteurization given by him is used in the preservation of food has a very important contribution on food microbiology. Pasteurization is defined as a process, which kills bacteria present in the liquid. He discovered on heating beer or wine kills most bacteria, which cause spoilage as well as prevents these beverages from souring. Pasteurization today is used in the dairy as well as the food industries for checking control of microbes and food preservation.
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